Production of High-Capacity Adenovirus Vectors

High-capacity adenoviral vectors (HC-Ad), also known as “helper-dependent” (HD-Ad), “gutless”, “gutted”, or “third-generation” Ad vectors, are devoid of all viral coding sequences and have shown promising potential for a wide variety of different applications—from classic gene therapy to genetic vaccination and tumor treatment. However, compared to first-generation adenoviral vectors their production is more complex and requires specific in-depth knowledge. This chapter delivers a detailed protocol for the successful production of HC-Ad vectors to high titers.