Quantitative In Vivo Microdialysis in Pharmacokinetic Studies
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Recent theoretical studies have yielded a more profound knowledge of the properties of recovery (the key parameter in quantitative
            microdialysis) and have put in evidence important limitations of the usual in vivo calibration methods used in quantitative
            microdialysis for pharmacokinetic studies. Recovery values obtained by using the more classical methods of calibration (the
            variation of flow rate perfusion method, the delivery and retrodialysis methods, and the no net flux method) can only be used
            to accurately convert dialysate drug concentrations into extracellular concentrations, when the drug of interest is in the
            body under steady-state conditions. Therefore, these in vivo calibration procedures must not be used when the drug studied
            has to be administered using modalities of administration which do not provide steady-state concentrations (for example, intragastric,
            subcutaneous, intraperitoneal, or intravenous bolus injections). The dynamic no net flux (DNNF) method, however, can be considered
            the only in vivo calibration method useful in PK experiments developed under transient conditions, although this calibration
            procedure has several serious disadvantages. The new modified version of the ultraslow microdialysis (the MetaQuant technique)
            overcomes many of the limitations of both the classical calibration and the DNNF methods and, therefore, it could be considered
            a promising tool in pharmacokinetics.
         
      









