Atomic Force Microscope-Enabled Studies of IntegrinExtracellular Matrix Interactions in Vascular Smooth Muscle and Endothelial Cells

The interactions between cell surface integrins and extracellular matrix (ECM) play important roles in the function of vascular smooth muscle and endothelial cells. Atomic force microscopy (AFM) has emerged as a powerful tool to mechanically engage cell surface integrins through functionalized probes, and to apply mechanical forces directly to cells or to specific protein–protein receptor ligand interactions, such as integrin–ECM interactions. In the example of integrins, this approach allows more accurate evaluation of the regulation of integrin adhesive activities, and provides a unique approach to access and investigate integrin-mediated cellular mechanical responses. In addition, the AFM is also useful for the measurement of the cell topographic features and cell and cytoskeletal mechanical properties, such as stiffness/elasticity.