Insertional Mutagenesis by Ty Elements in Saccharomyces cerevisiae
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The development of genetically marked Ty elements that transpose at high levels has made Ty mutagenesis a useful tool in yeast genetics (1 ,2 ). Ty mutagenesis is useful for several reasons:
| 1. |
Mutations made by insertion of a marked Ty element into a gene permit the rapid cloning of that gene into Escherichia coli .
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| 2. |
A tagged locus can be rapidly mapped to a chromosome by hybridization to chromosomes separated by electrophoresis.
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| 3. |
Mutations caused by Ty elements have useful phenotypes.
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