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        让人流口水的好书

        丁香园论坛

        974
        Protein–Protein Interactions :Methods and Applications March 2004

        http://biomed.humanapress.com/BookTOC.pasp?isbn=1-59259-762-9&returntoisbn=
        Part I. Overview

        Structural Basis of Protein–Protein Interactions
        Robert C. Liddington

        Quantitative Analysis of Protein–Protein Interactions
        Keith D. Wilkinson

        Part II. In Vitro Techniques

        Characterization of Protein–Protein Interactions by Isothermal Titration Calorimetry
        Adrian Velazquez-Campoy, Stephanie A. Leavitt, and Ernesto Freire

        Circular Dichroism Analysis for Protein–Protein Interactions
        Norma J. Greenfield

        Protein–Protein Interaction Analysis by Nuclear Magnetic Resonance Spectroscopy
        Guanghua Gao, Jason G. Williams, and Sharon L. Campbell

        Measuring Rhodopsin–G-Protein Interactions by Surface Plasmon Resonance
        John Northup

        Using Light Scattering to Determine the Stoichiometry of Protein Complexes
        Jeremy Mogridge

        Sedimentation Equilibrium Studies
        Ian A. Taylor, John F. Eccleston, and Katrin Rittinger

        Analysis of Protein–Protein Interactions by Simulation of Small-Zone Gel Filtration Chromatography
        Rosemarie Wilton, Elizabeth A. Myatt, and Fred J. Stevens

        Fluorescence Gel Retardation Assay to Detect Protein–Protein Interactions
        Sang-Hyun Park and Ronald T. Raines

        Fluorescence Polarization Assay to Quantify Protein–Protein Interactions
        Sang-Hyun Park and Ronald T. Raines

        Studying Protein–Protein Interactions via Blot Overlay or Far Western Blot
        Randy A. Hall

        Glutathione-S-Transferase–Fusion-Based Assays for Studying Protein–Protein Interactions
        Haris G. Vikis and Kun-Liang Guan

        Affinity Capillary Electrophoresis Analyses of Protein–Protein Interactions in Target-Directed Drug Discovery
        William E. Pierceall, Lixin Zhang, and Dallas E. Hughes

        Mapping Protein–Ligand Interactions by Hydroxyl-Radical Protein Footprinting
        Nick Loizos

        Use of Phage Display and Polyvalency to Design Inhibitors of Protein–Protein Interactions
        Michael Mourez and R. John Collier

        Part III. Detecting Protein–Protein Interactions in Heterologous Systems

        A Bacterial Two-Hybrid System Based on Transcriptional Activation
        Simon L. Dove and Ann Hochschild

        Using the Yeast Two-Hybrid System to Identify Interacting Proteins
        John Miller and Igor Stagljar

        Analysis of Protein–Protein Interactions Utilizing Dual Bait Yeast Two-Hybrid System
        Ilya G. Serebriiskii and Elena Kotova

        The Split-Ubiquitin Membrane-Based Yeast Two-Hybrid System
        Safia Thaminy, John Miller, and Igor Stagljar

        Reverse Two-Hybrid Techniques in the Yeast Saccharomyces cerevisiae
        Matthew A. Bennett, Jack F. Shern, and Richard A. Kahn

        Mammalian Two-Hybrid Assay for Detecting Protein–Protein Interactions In Vivo
        Jae Woon Lee and Soo-Kyung Lee

        Co-Immunoprecipitation from Transfected Cells
        Shane C. Masters

        Part IV: Probing Protein–Protein Interactions in Living Cells

        Microscopic Analysis of Fluorescence Resonance Energy Transfer (FRET)
        Brian Herman, R. V. Krishnan, and Victoria E. Centonze

        Monitoring Molecular Interactions in Living Cells Using Flow Cytometric Analysis of Fluorescence Resonance Energy Transfer
        Francis Ka-Ming Chan

        Fluorescence Correlation Spectroscopy: A New Tool for Quantification of Molecular Interactions
        Keith M. Berland

        Confocal Microscopy for Intracellular Co-localization of Proteins
        Toshiyuki Miyundefined*a~Kbr_~H~M~2~1~Kbr_~H~M~2~1Mapping_Biochemical_Networks_with_Protein~FFragment_Complementation_Assays~Kbr_~H~M~2~1Ingrid_Remy_and_Stephen_W._Michnick~Kbr_~H~M~2~1~Kbr_~H~M~2~1In_Vivo_Protein_Cross~FLinking~Kbr_~H~M~2~1Fabrice_Agou~E_Fei_Ye~E_and_Michel_Véron

        Part V. Proteomics-Based Approaches

        Computational Prediction of Protein–Protein Interactions
        John C. Obenauer and Michael B. Yaffe

        Affinity Methods for Phosphorylation-Dependent Interactions
        Greg Moorhead and Carol MacKintosh

        Two-Dimensional Gel Electrophoresis for Analysis of Protein Complexes
        Karin Barnouin

        Sample Preparation of Gel Electrophoretically Separated Protein Binding Partners for Analysis by Mass Spectrometry
        Rainer Cramer, Malcolm Saxton, and Karin Barnouin

        Quantitative Protein Analysis by Solid Phase Isotope Tagging and Mass Spectrometry
        Huilin Zhou, Rosemary Boyle, and Ruedi Aebersold

        Internet Resources for Studying Protein–Protein Interactions
        Shane C. Masters

        Index
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