• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Rapid DNA Preparation for Restriction Analysis

        互联网

        710

        SOLUTIONS:

        Green and blue solutions from Clone Checker kit (cat.no.11666-013).

        PROCEDURE:

        Place 3-6µl of an overnight culture in an Eppendorf tube (or spread part of a bacterial colony on the bottom of tube with a toothpick,which then can be used to create new colony on another plate).Add 8µl of green solution .Vortex

        Heat at 100℃ for 30 sec.,then cool to 4℃

        Add 2µl 10x restriction buffer,enzymes and water to the cell preparation to 20µl total.

        Incubate at the appropriate restriction enzyme temperature for 30 min.

        Add 2µl stop dye with RNase (blue solution ),mix and run on an agarose gel.

        (Note: if the gel is stained briefly after the run in ethidium bromide (0.5µg/ml),any genomic DNA smear may appear less prominent than if the gel were run in buffer containing ethidium bromide)

        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序