• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Direct Immunofluorescent Labeling of Cells

        互联网

        681
        In their original immunofluorescent labeling technique, Coons and colleagues employed a one-step, direct labeling of antigen using a fluorescein-conjugated antibody (1 , 2 ). Although this was not the first published report of a chemically modified antibody, it was the first time the antibody was used as a tool to examine an antigen in situ. Shortly thereafter, this method was adapted, giving rise to the indirect labeling technique (3 ). Rather than conjugating a marker molecule to the primary antibody, in this technique, a secondary antibody raised against the γ globulin of the primary species is conjugated with the marker molecule (Fig. 1 ). Although the direct labeling technique has the advantage of being quick, requiring a single incubation with the labeled reagent and one subsequent wash step, there are disadvantages. Each different primary antibody must be fluorescently labeled, and the resulting fluorescence is weak since only one labeled primary antibody binds to each antigen. The indirect labeling technique adds one more incubation and wash step, but it has the advantage of amplifying the fluorescent signal because several fluorescently labeled secondary antibodies can bind to each primary antibody. In addition, labeled secondary reagents specific for various species’ immunoglobulin classes are readily available commercially, stable in the lyophilized or frozen state, and relatively inexpensive.
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序