• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Immunocytochemical Techniques

        互联网

        666
        One of the most powerful tools in the identification of various neuronal types and then connectivities is immunocytochemical localization of specific neuronal markers, e g., synthetic enzymes for neurotransmitters at precise cellular and subcellular levels. Several methods exist for the localization of tissue antigens, at both the light and electron microscopic (EM) levels. The fluorescein-labeled antibody or immunofluorescent method developed by (1958 ) for the localization of tissue antigens with the light microscope has been employed for the localization of enzymes involved in the metabolism of neurotransmitters such as dopamine-β-hydroxylase (Hartman, et al., 1972 ; Hartman, 1973 ), tyrosine hydroxylase (Pickel et al., 1975a ), phenylethanolamine-N -methyltransferase (Hokfelt et al., 1974 ), DOPA decarboxylase (Hokfelt et al, 1973 ), and L-glutamate decarboxylase (GAD) (Kataoka et al., 1984). There are some deficiencies in this otherwise sensitive and specific method, however, such as the masking of the specific fluorescence by the inherent background fluorescence of tissue, and a lack of permanence of the preparations. (1966 ), (1966 ) reported that enzymes of small molecular weight, such as acid phosphatase or peroxidase, could be conjugated to antibodies by bifunctional reagents.
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序