Generation of Multiple Site-Specific Mutations by Polymerase Chain Reaction
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Generating mutant proteins has become a routine strategy for molecular and structural biologists, to understand the structure-function
relationship of a given protein of interest. In this, site-directed mutagenesis has proven to be a valuable technique. After
the advent of polymerase chain reaction (PCR), PCR-
mediated mutagenesis has been the method of choice to change from one amino acid to another, which has replaced the classic
Kunkel’s method (1
).
