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        Looking for the Niche: Substance Delivery into the Lateral Ventricle of the Brain: The Osmotic Minipump System

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        The infusion of different substances into the left ventricle of the brain allows substances to reach the subventricular zone, one of the neural stem cell niches in the adult brain. Implantation of an osmotic minipump delivers proteins, virus and drugs directly into the lateral ventricle to act locally. Here we describe this method consisting on a cannula implanted into the lateral ventricle and linked to an osmotic minipump via catheter. The cannula is fixed over the brain and the minipump is placed subcutaneously. This system can be maintained from days up to several weeks and ensures constant and regular delivery of the desired biological product.
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