Silver Staining of Acrylamide Gels
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Inc. Time |
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| 1. Gel Fix Solution (50% MeOH, 12% TCA, 2% CuCl2) |
20 min. |
|
| 100% MeOH |
250ml |
<center> (Good for 1 month)</center> |
| 100% TCA |
60ml |
|
| CuCl2 |
10g |
|
| H2O |
to 500ml |
|
| 2. Solution A (10% EtOH, 5% Acetic Acid) |
10 min. |
|
| 100% EtOH |
100ml |
|
| 100% Acetic Acid |
50ml |
|
| H20 |
850ml |
|
|
1000ml |
||
| 3. 0.01% KMnO4 |
10 min. |
|
| KMnO4 |
0.02g |
|
| dH2O |
200ml |
|
|
200ml |
||
| 4. Solution A (from Above) |
10 min. |
|
| 5. 10% EtOH |
5 min. |
|
| 6. dH2O |
5 min. |
|
| 7. 0.1% AgNO3 |
10 min. |
|
| AgNO3 |
0.2g |
|
| dH2O |
200ml |
|
|
200ml |
||
| 8. dH2O dip |
20 sec. |
|
| 9. 10%K2CO3 |
1 min. |
|
| 10. Developer (2% K2CO3, 0.01% Formaldehyde) |
until developed |
|
| 10% K2CO3 |
40ml |
|
| dH2O |
160ml |
|
| 37% Formaldehyde |
54ul |
|
|
200ml |
||
| 11. Solution A (from above) |
1-2 min. |
|
| 12. H2O |
15 min |
|
Comments: |
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