• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        CELLULAR ELECTRON MICROSCOPY

        互联网

        1381

        Description

        Recent advances in the imaging technique electron microscopy (EM)have improved the method,making it more reliable and rewarding,particularly in its description of three-dimensional detail。This book will help biologists from many disciplines understand modern EM and the value it might bring to their own work。The book?s five sections deal with all major issues in EM of cells: specimen preparation,imaging in 3-D,imaging and understanding frozen-hydrated samples,labeling macromolecules,and analyzing EM data。Each chapter was written by scientists who are among the best in their field,and some chapters provide multiple points of view on the issues they discuss。Each section of the book is preceded by an introduction,which should help newcomers understand the subject。The book shows why many biologists believe that modern EM will forge the link between light microscopy of live cells and atomic resolution studies of isolated macromolecules,helping us toward the goal of an atomic resolution understanding of living systems.

        全书分4部分

        1.Preparing Cells for Electron Microscopy
          2.Imaging Fixed Cells in Three Dimensions
          3.Imaging Frozen‐Hydrated Cells and Cell Parts
          4.Localizing Macromolecules in Cells
          5.Aspects of Data Collection and Analysis

        Introduction: McIntosh - A new era in electron microscopy of cells

        Preparative Methods for Cellular Electron Microscopy

        1.The physics of rapid cooling and its implications for cryo-immobilization of cells
          2.Cryopreparation Methods for Electron Microscopy of Selected Model Systems
          3.Cryo-preparation methodology for plant cell biology
          4.Correlative Light and Electron Microscopy of Early C。elegans Embryos in Mitosis

        Imaging Fixed Cells in Three Dimensions

        5.Understanding microtubule organizing centers by comparing mutant and wild-type structures with electron tomography
          6.Whole Cell Investigation of Microtubule Cytoskeleton Architecture by Electron Tomography
          7.Electron Microscopy of archaea
          8.Reconstructing Mammalian Membrane Architecture by Large Area Cellular Tomography
          9.Visualization of Membrane-Cytoskeletal Interactions during Plant Cytokinesis
          10.Electron Tomographic Methods for Studying the Chemical Synapse
          11.Using electron microscopy to understand functional mechanisms of chromosome alignment on the mitotic spindle
          12.EM Analysis of the Leading Edge in Migrating Cells
          13.Imaging actomyosin in situ

        Imaging Frozen-Hydrated Cells and Cell Parts

        14.Electron tomography of bacterial chemotaxis receptor assemblies
          15.How to “read” a vitreous section
          16.Single Particle Electron Cryomicroscopy of the Ion Channels in the Excitation-Contraction Coupling Junction
          17.Electron microscopy of microtubule-based cytoskeletal machinery
          18.Reconstructing the endocytotic machinery

        Localizing Macromolecules in Cells

        19.3-D Immuno-localization with plastic sections
          20.EM analysis of viral morphogenesis
          21.Electron tomography of immuno-labeled cryosections
          22.Visualizing macromolecules with fluoronanogold: from photon microscopy to electron tomography
          23.Markers for correlated light and electron microscopy
          24.Localizing Specific Elements Bound to Macromolecules by EFTEM
          25.Localization of protein complexes by pattern recognition

        Aspects of Data Collection and Analysis Instrumentation for image acquisition

        26.Application of Energy Filtered Electron Microscopy to Tomography of Thick,Selectively Stained Biological Samples
          27.Optimization of Image Collection for Cellular Electron Microscopy
          28.Future Directions for Camera Systems in Electron Microscopy

        Computational methods for the analysis of cellular data

        29.Structure determination in situ by averaging of tomograms
          30.Methods for image segmentation in cellular tomography
          31.Database Resources for Cellular Electron Microscopy

        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序