Assessing Microvessels After Spinal Cord Injury
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This chapter focuses on the assessment of spinal cord microvessels which are lost at the injury epicenter within 24 h after spinal cord contusion or compression in adult rats and mice. The penumbral blood vessels undergo angiogenesis during the first and second week possibly contributing to recovery. Rescue of blood vessels results in better tissue preservation and functional outcomes. We describe various markers, intravenous labeling techniques, quantitative histological measurements, permeability assays, collection of purified microvessels, genetic mouse models, and endothelial cell culture methods to assess vascular status after spinal cord injury.
