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        Analysis of the Cell Cycle in Mouse Embryonic Stem Cells

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        The molecular mechanisms underlying self-renewal of pluripotent embryonic stem (ES) cells is still poorly understood. Deciphering these mechanisms is of prime importance for at least two reasons: (1) ES cells derive from, and are closely related to, the pluripotent stem cells of the blastocyst, the founder cells of the whole embryo proper. Hence, they constitute a unique model for studying embryonic development at the time of implantation, when embryos are inacessible to experimental manipulation; and (2) Isolating and manipulating ES cells in species of economic or therapeutic interests is more difficult than in the mouse. It is likely that better defining their growth requirements will lead to major improvements in their culture conditions.
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