Solubilization of Aggregates Formed by Expanded Polyglutamine Tract Expression in Cultured Cells

互联网2014-02-10

489

The expression of expanded polyglutamine in mammalian cells causes the formation of aggregates. For elucidation of the biochemical properties of the aggregates, isolation and solubilization of the aggregates are required. This chapter provides useful protocols for the solubilization of polyglutamine aggregates and further applications of protein analysis.

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