• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Rho GTPase Activity Analysis in Plant Cells

        互联网

        889
        Rho-family small GTPases are conserved molecular switches of signaling networks in eukaryotic cells, and regulate many cellular responses, such as cytoskeletal reorganization, gene expression, and polarized vesicular trafficking. To understand the functions of Rho GTPase, it is important to investigate how the activity of Rho GTPase is regulated. Plant Rho-like GTPases (ROPs) are known to be regulated by hormones and environmental cues. A rapid activation of ROPs by a stimulus implies a direct signaling role for the ROP GTPases. Here, I describe an ROP activity assay that allows the measurement of ROP GTPase activity that occurs within seconds upon treatment with a stimulus. This method has been successfully used to investigate auxin activation of ROPs in plant cells and will be generally useful for measuring changes in ROP activity with high time resolution (Xu et al., Cell 143:99–110, 2010).
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序