Transient Transfection of Human Retinoblastoma Cells: Application to the Analysis of the Regulatory Regions of Photoreceptor-Specific Genes

Retinoblastoma (Rb) is an intraocular tumor usually diagnosed in children under four years of age (1 ). The tumor rises when both alleles of the Rb tumor suppressor gene become inactivated in a retinal precursor cell during development (2 ,3 ). The first retinoblastoma cell line to be established in culture, Y-79 (4 ), has been shown to originate from neuroectodermal cells that express both neuronal and glial cell markers (3 ). Both Y-79 cells and Rb tumor cells produce mRNAs encoding several proteins unique to the photoreceptors (5 ), including different subunits of cone- and rod-specific cGMP-phosphodiesterases (6 ). Therefore, cultured Y-79 cells, which have a human retinal origin, could be particularly useful for studying the regulatory mechanisms of photoreceptor-specific gene expression (7 ).