Analyzing how Cell Adhesion Controls Mammary Gland Function by Transplantation of Embryonic Mammary Tissue from Knockout Mice

Understanding how cell adhesion to the extracellular matrix controls mammalian development has been explored extensively using gene knockout technology. However, in some knockout mice, animals die during late embryogenesis or shortly after birth. In such cases, it is possible to analyze embryonic developmental phenotypes, but it is less easy to determine the in vivo role of cell–matrix interactions in adult tissues. Although this problem has been partially solved by the development of tissue-specific knockouts, the approach relies on appropriate tissue-specific promoters. In many cases, genes that uniquely characterize specific cell types within complex tissues have not been identified. Thus, knockout technology can be restrictive when analyzing cell–matrix interactions in specific cases of tissue development and/or homeostasis. Here we describe how transplantation of mammary tissue into recipient hosts can be used to extend the understanding of cell adhesion functions in developmental processes.