Acquisition, Display, and Analysis of Digital Three-Dimensional Time-Lapse (Four-Dimensional) Data Sets Using Free Software Applications
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The ability to capture and visualize information throughout the volume of a living three-dimensional (3-D) specimen as it changes over time (four-dimensional [4-D] imaging) has been the goal of many biological researchers. In practice, this is usually accomplished by gathering a series of 3-D data sets or “timepoints” from a given sample where time is allowed to pass between each 3-D acquisition.
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