Massive Indexed Parallel Identification of Transposon Flanking Sequences
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		The large scale sequencing of insertion element flanking sequences has revolutionized reverse genetics in plant research: Insertion mutants can now simply be identified in silico by BLAST searching the resulting flanking sequence databases. The development of next-generation sequencing technologies has further facilitated the creation of flanking sequence collections derived from entire mutant populations. Here we describe a highly efficient and widely applicable method that we developed to amplify, sequence, and identify dTph1 transposon flanking sequences from a library of 1000 Petunia W138 individuals simultaneously.




![Npy/Npy蛋白Recombinant Mouse Pro-neuropeptide Y (Npy)重组蛋白Pro-neuropeptide Y [Cleaved into: Neuropeptide Y(Neuropeptide tyrosine)(NPY); C-flanking peptide of NPY(CPON)]蛋白](https://img1.dxycdn.com/p/s14/2024/0914/795/0844698182989384381.jpg!wh200)






