• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Neocortical Circuit Interrogation with Optogenetics

        互联网

        657
        Optogenetic tools have gained popularity for enabling manipulation of specified populations of neurons on a precise temporal scale. These opsins, as the proteins are known, utilize retinal (Vitamin A) as a co-factor—in a form related to the same molecule used by the human retina as a light-sensing moiety; opsin function does not require (at least in vertebrates) exogenous supplementation of this molecule [1]. Taken together, these properties of optogenetic tools situate them ideally for the interrogation of neural circuits in the settings of acute slice preparations, in vivo single and multiunit recordings, and behaving animals. Robust and diverse tools now exist to allow for both gain and loss of function experiments in all of these settings; for example, recent engineering and genomic approaches have introduced diversity in the spectral properties of both excitatory and inhibitory opsins, opening the door to combinatorial optogenetic experiments and simultaneous manipulation of multiple populations of neurons in the same physical volume [2] (see Chapter 5 in this volume). Here we discuss circuit-targeting strategies with examples from the recent literature, focusing particularly on approaches and results in the study of mammalian neocortex.
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序