Histological Characterization of Physiologically Determined Fast-Spiking Interneurons in Slices of Primate Dorsolateral Prefrontal Cortex
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GABA (γ-aminobutyric acid)-ergic interneurons are a structurally and functionally diverse group of cells that, together, regulate
the activity of neuronal networks giving rise to the brain oscillations necessary for information processing. The phenotypes
of GABAergic neurons can be classified by morphological, �electrophysiological, and neurochemical features. However, as further
information is gathered about these features and the role(s) each phenotype plays in shaping cortical function, group membership
will undoubtedly change. This chapter details a set of methodologies that can be used to characterize GABAergic �neurons in
the monkey dorsolateral prefrontal cortex (DLPFC). Specifically, it (1)
provides a brief protocol for the dissection of DLPFC tissue from monkeys, (2) describes electrophysiological measures used
to identify GABAergic neurons, (3) details how to fill neurons for morphological analyses, and (4) describes a fluorescence
imaging technique for the quantification of fluorescently labeled puncta (putative synaptic components), the colocalization
of different labels in the same synaptic structure, and the quantitation of fluorescence intensity in these same structures
in brain tissue sections.
