Yeast Two-Hybrid System Screening

The yeast two-hybrid system is a poau]werful molecular genetic tool conceived by Fields and Song (1 ). The article is a comprehensive set of methods designed to take the reader through a yeast two-hybrid analysis of your favorite gene (YFG). This article details the preparation for a screen, the screen itself, as well as the analysis of the positives identified. Using these methods, the readers should be able to successfully negotiate a yeast two-hybrid screen using the Fields’ or related systems.