In Situ Hybridization as a Tool to Study the Role of MicroRNAs in Plant Development

MicroRNAs (miRNAs) have a vital role in the generation of plant forms through posttranscriptional regulation of the accumulation of developmental regulators. Analysis of their roles requires detailed knowledge of their expression patterns. We describe an in situ hybridization technique we have used to study the patterns of miRNA accumulation in Arabidopsis and in maize.