Identification of GPCR Localization in Detergent Resistant Membranes

Lipid domains of the plasma membrane were originally described as a cell matrix insoluble in cold �nonionic detergents and enriched in glycosphingolipids. Because of these biochemical properties, these membrane domains were termed d etergent-r esistant m embranes (DRMs) or d etergent-i nsoluble �g lycolipid-enriched (DIG) membranes. Membrane rafts and caveolae are two types of lipid domains that share these properties, as well as structural/functional dependence on membrane cholesterol. Membrane rafts and caveolae are believed to act as signaling platforms for ligand-activated receptors, thereby contributing to the regulation of receptor function. Here we describe a simple method to assess the association of GPCRs with detergent resistant membranes in native brain tissue and cultured cells.