PLANTING SEED
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with tweezers- recommended for pots:
1. Wet small tweezer tips with dH2 0.
2. Touch one tip to a seed.
3. Move seed to soil by touching tweezer tip to desired spot on soil.
4. Cover pots with humidomes. Let pots sit in 4°C room for 2 days and then move up to growth room.
with 0.1% agar- recommended for flats
1. Weigh out seed for planting. 2mg of seed = 100seed. Suspend each 2-3mg of seed in 5ml agar. Planting more than 200 seed/flat may make the plants less happy.
2. Suspend seeds in agar.
3. Disperse seeds over soil with a disposable Pasteur pipet.
4. Cover flats with humidomes. Let flats sit in 4°C room for 2 days and then move up to growth room.
<center> <p> </p> </center>上一篇:Pollen Tube Germination Media 下一篇:GUS Staining of Pollen


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