An Immuno-Slot-Blot Assay for Detection and Quantitation of Alkyldeoxyguanosines in DNA

The detection and quantitation of DNA adducts plays a central role in the determination of dose-response relationships for chemical carcinogens, mutagens, and chemotherapeutic agents in experimental laboratory investigations. Furthermore, it serves in molecular dosimetry and risk estimation of humans chronically exposed to environmental carcinogens, and of cancer patients undergoing treatment with alkylating cytostatic drugs (1 ,2 ). These investigations require sensitive methods that do not necessitate the administration of radioactively labeled compounds.