Analysis of Drug Uptake-Flow Cytometry

Flow cytometry is a laser-based technique used for the quantitation of specific intracellular and extracellular properties of cells, bacteria, or other biological particles. It is unique in its ability to perform simultaneous multiparameter analysis and to sort single cell types from heterogeneous mixtures. Its application initiated the third phase of method development in modern cell biology, following the introduction of microspectrophotometry and autoradiography (1 ). In comparison with other methodologies, flow cytometry offers very rapid measurements with hundreds or even thousands of cells being analyzed in 1 s with high accuracy and reproducibility.