Agrobacterium-Mediated Transformation of Stem Disks from Oilseed Rape (Brassica napus L.)
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The commercial importance of oilseed rape (Brassica nupus L.) makes it desirable to have an efficient transformation protocol for practical applications. With the exception of a few reports, transformation experiments with B. napus using Agrobacterium tumefaciens yielding transgenic plants have been performed principally with the spring variety B. napus L. ssp. oleifera cv. Westar (1 –5 ). Transformation protocols are relatively specific for cv. Westar and cannot be extended to most of the other spring and none of the winter varieties tested (6 ). There are only isolated reports on transgenic winter oilseed rape plants (6 –8 ).
