Regulation of MAP Kinase Signaling by Calcium

Mitogen-activated protein kinase (MAPK) signaling influences a variety of cellular responses, ranging from stimulation of cell proliferation to induction of senescence and/or apoptosis. Ca²⁺ is a ubiquitous intracellular signaling molecule that controls multiple processes in cells. Published evidence has identified both direct and indirect interactions between the Ca²⁺ and MAPK signaling pathways. Here, we describe assays to accurately determine the effect of changes in intracellular Ca²⁺ concentration on MAPK activation.