Electrophoretic Mobility Shift Assays for the Analysis of DNAProtein Interactions

Electromobility shift assay is a simple, efficient, and rapid method for the study of specific DNA–protein interactions. It relies on the reduction in the electrophoretic mobility conferred to a DNA fragment by an interacting protein. The technique is suitable to qualitative, quantitative, and kinetic analyses. It can also be used to analyze conformational changes.