Mitochondrial Proteomics: Analysis of a Whole Mitochondrial Extract with Two-Dimensional Electrophoresis
互联网
509
Mitochondria are complex organelles, and their proteomics analysis requires a combination of techniques. The emphasis in this
            chapter is made first on mitochondria preparation from cultured mammalian cells, then on the separation of the mitochondrial
            proteins with two-dimensional electrophoresis (2DE), showing some adjustment over the classical techniques to improve resolution
            of the mitochondrial proteins. This covers both the protein solubilization, the electrophoretic part per se, and the protein
            detection on the gels, which makes the interface with the protein identification part relying on mass spectrometry.
         
      









