Building a High-Density Genetic Map Using the AFLP Technology

The AFLP⁻ (Keygene, Wageningen, The Netherlands) technology allows the simultaneous amplification of many restriction fragments in a single polymerase chain reaction (PCR). Thus, the technology can be used to very efficiently detect restriction fragment polymorphisms. As an example of the vast numbers of markers that can be detected, the author describes here a protocol to construct a high-density genetic map mArabldopsis . The map may be used to identify primer combinations that will target fragments mapped to a specific area of the genome.