FROGGING
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FROGGING
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- Frog is 8x6 prong. keep in EtOH, flame, then dip in sterile dH2 O before use.
- Dip & swirl into yeast diluted in microtiter plates.
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Place on dry plate 1-2sec, remove, will leave 1-2 ul.
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Serial dilutions (in steril water on YPD) -8 samples x 6 dilutions. Aim for initial dilution at ~OD 0.1 [850 cells/uL] in 250 ul. 5X dilutions (do 4) 50 ul into 200 ul H2 O
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Check plates, sometimes not even. Try pressing on FROG
OD600 [cells/mL] cells/1 ul spot 5X dilution ~0.1 85x104 850 850 28x104 283 170 9.4 94 34 3.2 32 6.8 1 11 0.35 3.5
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