lmmunocytochemical Methods for Regulatory Peptides
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Immunocytochemistry employs antibodies to detect peptides or proteins in tissue preparations (1
). However, this in itself is not a visible reaction. Several methods, employing secondary antibodies labeled in some way
to render them visible, generally with fluorescent compounds or enzymes fed with suitable chromogenic substrates, are in current
use (2
). The method chosen depends on a number of factors. The greater sensitivity of enzyme methods is rarely an advantage in the
immunocytochemical demonstration of regulatory peptides, as these substances tend to occur at relatively high concentrations
in tissues. Advantages of enzyme methods are that the preparations are permanent and the underlying tissue morphology is available
for study along with the labeled antigen. This is of particular importance in diagnostic histopathology.
