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        Visually Guided Patch-Clamp Recordings in Brain Slices

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        Brain slices have become an integral part of synaptic and cellular physiology since the pioneering studies of Henry McIlwain (Li and McIlwain, 1957 ; Yamamoto and McIlwain, 1966 ). The hippocampal slice preparation was first brought to the United States from Per Anderson’s lab, initially by Tim Teyler and followed shortly after by Phil Schwartzkroin. Over the years the development of the in vitro brain slice preparation has enabled electrophysiologists to study various aspects of the nervous system in an isolated preparation that still retains many of the brain’s complement of neuronal connections. Through the use of brain slice preparations, much has been learned about the intrinsic properties and morphology of different populations of neurons, the connectivity between different cell types within or between brain regions, the quantal nature of transmitter release, and the various forms of synaptic plasticity. This section focuses on some of the important aspects of preparing and maintaining healthy brain slices, along with some of the rationales for selecting certain procedures.
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