• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Analysis of CFTR Endocytosis by Cell Surface Biotinylation

        互联网

        446
        Correct localization of cystic fibrosis transmembrane conductance regulator (CFTR) is critical to its function. Although an intracellular role for CFTR is still somewhat controversial (1 ), there is clear agreement on an important role for CFTR in the plasma membrane. However, it is not only important that CFTR be inserted into the plasma membrane, it has to stay there the requisite amount of time. Although many CFTR mutations result in CFTR molecules that reach the plasma membrane (with altered conductance or regulation), the most clinically important mutation, AF508 CFTR, fails to reach the plasma membrane in physiologically relevant quantities. The precise amount of AF508 CFTR that reaches the plasma membrane remains controversial (2 ,3 ). Even so, until the “holy grail” of gene therapy is realized, therapeutic strategies to augment plasma membrane expression of mutant CFTR remain important. Indeed, many laboratories, both academic and commercial, are currently engaged in screening endeavors to identify pharmacological agents capable of improving the folding and traffic of mutant CFTR molecules (especially AF508 CFTR) leading to their insertion into the plasma membrane.
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序