Selection of Yeasts Hybrids Obtained by Protoplast Fusion and Mating, by Differential Staining, and by Flow Cytometry

Chapter 38 deals with methods for hybridization of yeasts in different genera by protoplast fusion and for selection of hybrids by double-fluorescence staining and use of a micromanipulator. This chapter describes a similar but automated and rapid procedure for selection of hybrids; flow cytometry and cell sorting are used (1 ). Mating, instead of fusion, followed by flow sorting as is done to obtain hybrids (2 ) also is described, together with some modifications.