• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Overview of Fluorochromes

        互联网

        740
        Since its inception in the 1940s, the technique of immunofluorescence microscopy has provided a sensitive, high-resolution method for determining the presence of, and distribution of, an antigen within a specimen. Fluorescent molecules, termed fluorochromes, can be conjugated directly to antibodies by covalent linkage, or coupled indirectly to antibodies via conjugation to proteins A and G or through an avidin-biotin bridge (see Chapters 6, 7, and 25). A fluorochrome coupled to an antibody or other probe may be termed a fluorophore; here, the term fluorochrome is used throughout. The basic features of immunofluorescence are straightforward, but a working knowledge of the commonly used fluorochromes is of value in obtaining maximum performance from immunofluorescence microscopy and flow cytometry. The discussion below focuses on fluorochromes commonly used for immunolabeling, and does not encompass fluorochromes that provide molecule- or organelle-specific labeling without the use of antibodies.
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序