Analysis of Bulge Stem Cells from the Epidermis Using Flow Cytometry
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The epidermis is a multilayered epithelium consisting of multiple different progenitor cell populations, all of which are
important to epidermal function. In order to study these populations, several techniques have been developed that enable specific
purification of the different progenitor cell populations. The best characterized stem cell population in the epidermis, and
likely the most pluripotent, are the quiescent stem cells in the hair follicle bulge. In this chapter, we provide a method
for isolating bulge stem cells from skin of adult mice using fluorescence-activated cell sorting of immunofluorescently labeled
keratinocytes. We use the cell surface markers CD34 and α6-integrin for the enrichment of bulge stem cells. This method also
contains notes on how to adjust the cytometer settings for a reproducible analysis.
