• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Construction of Retro viral Packaging Cell Lines

        互联网

        664
        Most of the time, retrovirus vectors retain only cis -acting sequences from the original viral genome. These sequences allow the recombinant structure to be transcribed (LTR promoter/enhancer) and the RNA to be processed (splicing and polyadenylation signals), packaged into a virion particle (packaging sequences), and replicated by the reverse transcriptase (tRNA binding site, R region, and polypurine track). The other viral functions have to be provided in trans for the assembly of recombinant viral particles to take place. This can be simply achieved by using a replication-competent helper virus, leading to the production of a mixed population. Nevertheless, helper-free stocks are desirable for most applications, since
        1.  The high frequency of recombination in a mixed virus stock is likely to lead to the appearance of recombinants with unknown structure and activity. These new chimeras, either spread by the helper virus or replication-competent themselves, create a potential safety problem.
        2.  Cell lineage analysis using retroviral marking can only be performed and interpreted in a helper-free context
        3.  In vivo gene transfer experiments can be jeopardized by disease (s) associated with helper-virus infection (1 ).
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序