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        分子生物学常用实验技术经典介绍与指南大全

        丁香园论坛

        2901
        一、《Molecular Cloning 3》

        英文版,主要介绍分子克隆的基本实验技术操作,新手如果看不明白此版,可以自己买一本军科院翻译的中文版。

        Table of Contents
        Chapter 1: Plasmids and Their Usefulness in Molecular Cloning
        Chapter 2: Bacteriophage and Its Vectors
        Chapter 3: Working with Bacteriophage M13 Vectors
        Chapter 4: Working with High-Capacity Vectors
        Chapter 5: Gel Electrophoresis of DNA and Pulsed-Field Agarose
        Chapter 6: Preparation and Analysis of Eukaryotic Genomic DNA
        Chapter 7: Extraction, Purification, and Analysis of mRNA from Eukaryotic Cells
        Chapter 8: In Vitro Amplification of DNA by the Polymerase Chain Reaction
        Chapter 9: Preparation of Radiolabeled DNA and RNA Probes
        Chapter 10: Working with Synthetic Oligonucleotide Probes
        Chapter 11: Preparation of cDNA Libraries and Gene Identification
        Chapter 12: DNA Sequencing
        Chapter 13: Mutagenesis
        Chapter 14: Screening Expression Libraries
        Chapter 15: Expression of Cloned Genes in Escherichia coli
        Chapter 16: Introducing Cloned Genes into Cultured Mammalian Cells
        Chapter 17: Analysis of Gene Expression in Cultured Mammalian Cells
        Chapter 18: Protein Interaction Technologies

        http://www.promega.com/guides/subcloning_guide/Subcloning_ntbk.pdf

         

        三、Beginning Molecular Biology Laboratory Gudie

        新手入门的好教材,非常全面,非常亲切。值得收藏。
        http://www.research.umbc.edu/~jwolf/method1.html

        内容介绍:

        CHAPTER 1: General Laboratory Methods

        Safety Procedures
        Preparation of Solutions
        Disposal of Buffers and Chemicals
        Equipment Micropipets

        Using a pH meter
        Autoclave operating procedures
        Operating instructions for spectrophotometer

        Working with DNA
        Sterile Technique
        CHAPTER 2: Instructions for Notebook Keeping

        CHAPTER 3: Computer User's Guide

        UNIX commands
        File Transfer
        The World Wide Web
        The Wisconsin Package - gcg.
        CHAPTER 4: Molecular Biology Methods
        M.1: Preparation of genomic DNA from bacteria
        M.2: PCR amplification of DNA
        M.3: Restriction enzyme digestion of DNA
        M.4: Phenol/chloroform extraction of DNA
        M.5: Ethanol precipitation of DNA
        M.6: Agarose gel electrophoresis
        M.7: Transformation of E. coli by electroporation
        M.8: Wizard PCR preps DNA purification system
        M.9: Alternate method for purifying DNA from agarose gels
        M.10: Transfection of mammalian cells using Lipofectamine (LTI)
        M.11: Southern blotting
        M.12: RT-PCR Protocol
        M.13: Preparation of sequencing gels
        M.14: Isolation of RNA from mammalian cells using RNAZOL (Teltest)

        CHAPTER 5: Tissue Culture Methods
        Types of cells grown in culture
        Work area and equipment
        Preservation and storage
        Maintenance
        Safety considerations
        Tissue culture methods
        Determining cell counts

         

        四、Cloning Enzymes

        Cloning Enzymes, one in the Enzyme Resource Guide series, highlights those enzymes important in nucleic acid cloning procedures. Enzymes that modify nucleic acids provide the foundation for many molecular biology techniques: enzymes that are used to synthesize, degrade, join or remove portions of nucleic acids in a controlled and generally defined manner. Specific features of the in vivo functions of these enzymes have been exploited in vitro to provide many of the protocols currently used in nucleic acid manipulations.This guide mainly is targeted Ligases, Kinases and Phosphatases.

        Table of Contents Page

        Enzyme Activities Diagram
        The Cloning Enzymes
        Gene Cloning
        Ligases
        Kinases and Phosphatases
        RecA Protein and AgarACE® Enzyme
        Technical Appendix

        http://www.promega.com/guides/cloning_guide/cloningenz.pdf

         

        五、Nucleic Acid Purification Systems

        The isolation and purification of DNA is crucial to many applications in molecular biology and techonogy. Over the past decade, the purification of DNA has evolved from a multi-step process involving organic chemicals to a much simpler process, that is safer and faster. Promega continues to develop new DNA purification products to meet the diverse and changing needs of today's life science researcher. This Nucleic Acid Purification Systems guide presents our many new and innovative products for use in DNA and RNA purification.

        Table of Contents Page

        Genomic DNA
        Plasmid DNA
        Fragment DNA
        Total RNA

        http://www.promega.com/guides/nadp_guide/napd_guide.pdf

         

        六、 RNA Analysis Notebook

        RNA analysis is the starting point for many molecular biology procedures. The RNA Analysis Notebook provides an introduction to RNA procedures such as purifying RNA, amplifying via RT-PCR, making RNA in vitro and analyzing RNA by microarray. In addition, the RNA Analysis Notebook highlights products for performing these procedures.

        Table of Contents
        Working with RNA
        Purifying RNA and mRNA
        Amplifying RNA with RT-PCR
        Analyzing RNA with Microarrays
        Making RNA in vitro
        Silencing RNA in vivo (RNAi)

        http://www.promega.com/guides/rna_guide/rna_gde.pdf

         

        七、DNA Analysis Notebook

        DNA analysis is the starting point for many molecular biology procedures. The DNA Analysis Notebook provides an introduction to DNA procedures such as purifying genomic DNA, amplifying via PCR, retrieving DNA from PCR and cloning PCR DNA. In addition, the DNA Analysis Notebook features Promega products for performing these procedures.

        Contents

        Table of Contents
        Genomic DNA Purification
        Amplifying DNA
        PCR Clean-Up
        Cloning PCR DNA
        DNA Analysis Tools

        http://www.promega.com/guides/dna_guide/dna_guide.pdf

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        分子生物学常用实验技术经典介绍与指南大全

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