Identification of Lymphoma-Associated Antigens Using SEREX
互联网
703
Serological analysis of recombinant complementary deoxyribonucleic acid (cDNA) expression libraries (SEREX) is a powerful approach to identify immunogenic cancer-associated proteins using antibodies that are naturally present in the serum of cancer patients. This technique involves the screening of a relevant cDNA expression library with patient serum that has been cleaned to remove any antibodies that may recognize bacterial and/or viral proteins. Once antigens have been identified and their reactivity has been confirmed with a second round of screening, the gene encoding the protein can be sequenced and identified. Antigens can then be screened with a panel of allogenic sera from other patients and control individuals. This identifies disease-specific antigens, which may be useful diagnostic markers or, alternatively, targets for immunotherapy. This chapter describes the SEREX methodology in full.
![PARP9/PARP9蛋白/ADP-ribosyltransferase diphtheria toxin-like 9
B aggressive lymphoma protein
Poly [ADP-ribose] polymerase 9蛋白/Recombinant Human Poly [ADP-ribose] polymerase 9 (PARP9), partial重组蛋白](https://img1.dxycdn.com/p/s14/2024/0807/931/4874189879496701281.jpg!wh200)
