DNA Extraction from Cheek Cells

Extraction from Cheek Cells DNA

Sample Preparation

Cheek cells are obtained by rinsing the mouth with 25mls of any commercial mouth wash solution available for about 30sec the first thing in the morning. It is important not to brush your teeth before collecting the specimen. The mouth wash is then discarded into a sterile conical tube and sent to the laboratory.

A Salting Out Procedure for DNA extraction from Cheek Cells  
 

Fig.1. Amplification of CTLA4 gene by PCR. Tracks marked C using DNA extracted from cheek cells, the rest DNA extracted from peripheral blood leucocytes (WB).