Immunoblot Analysis and Band Depletion Assays
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Western blotting has been widely utilized to detect various polypeptides or polypeptide epitopes (e.g., posttranslational modifications) within cells (reviewed in 1 -4 ). If the signals in samples being analyzed are compared to a suitable standard curve and appropriate internal standards are utilized to confirm equivalent loading of various samples, Western blotting appears to be a suitable method of quantitating polypeptides as well.
