Flow Cytometric Analysis of Tumor Suppressor Gene-Induced Apoptosis

As interest in cell death has recently increased, many new techniques have been developed to detect and quantitate apoptosis in cells. Multiparameter flow cytometric-based assays provide statistical information on measured parameters at the single-cell level, and are therefore well suited for measuring apoptosis (1 ). Since apoptosis has many functional expressions, it is often desirable for investigators to demonstrate cell death by several different methods. A sampling of flow cytometric assays used in our laboratory is presented here.