Ribozyme-Mediated Trans Insertion-Splicing into Target RNAs
互联网
723
The trans insertion-splicing (TIS) reaction is a technique that can be used to site-specifically insert an RNA donor substrate into a separate RNA acceptor substrate. The TIS reaction, which is catalyzed by a group I intron-derived ribozyme from Pneumocystis carinii , is described with regards to system design, ribozyme preparation, and the overall protocol for conducting the TIS reaction.
![methyl trans-4-[5-(difluoromethyl)-4-nitro-pyrazol-1-yl]cyclohexanecarboxylate，2434852-20-5，≥97%，阿拉丁](https://img1.dxycdn.com/p/s14/2024/0619/706/8427599713567523081.jpg!wh200)
