Ribozyme-Mediated Trans Insertion-Splicing into Target RNAs
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The trans insertion-splicing (TIS) reaction is a technique that can be used to site-specifically insert an RNA donor substrate into a separate RNA acceptor substrate. The TIS reaction, which is catalyzed by a group I intron-derived ribozyme from Pneumocystis carinii , is described with regards to system design, ribozyme preparation, and the overall protocol for conducting the TIS reaction.
