• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        How to Study Pathologic Phenotypes of Knockout Mice

        互联网

        686
        It has become increasingly clear that when one knocks out a gene it is almost always impossible to predict the resultant phenotype. One must be prepared to look at every organ at every stage of development, from earliest embryonic life to old age. With any luck the knockout (KO) mice will have an obvious phenotype, such as an absent tail. Often the KO mice will have no apparent phenotype or will sicken and die prenatally, right after birth, during the first days or weeks of life, or later in life. The phenotype is critical. Thus, if a mutant is found to have no corpus callosum, that discovery can be published even if no one has figured out why the pups die shortly after birth (1 ). On the other hand, no one is interested in a mutant that simply dies or that has no phenotype. A common occurence is to make or acquire a second KO and to breed it with the first KO mice to make a double mutant. Sometimes breeding the mutated gene onto another genetic background by repeated backcrossings (i.e., making a new congenic strain) brings out a phenotype, as many genes are now known to be or are suspected to be modified by other genes.
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序