• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Probing Kinetochore Structure and Function Using Xenopus laevis Frog Egg Extracts

        互联网

        549
        Kinetochores are multiprotein machines that initiate mitotic checkpoint signaling and control chromosome movement through interactions with microtubules. Our lab has utilized Xenopus laevis frog egg extracts to investigate the requirements for kinetochore assembly and disassembly in vertebrates. Egg extracts support the assembly of functional kinetochores that are capable of binding microtubules, aligning and segregating chromosomes, and sending spindle checkpoint signals. This is the only in vitro system that assembles functional kinetochores, making it particularly well suited for these types of studies. Probing kinetochore assembly using the biochemically tractable egg extract system has elucidated the intricate assembly requirements for numerous vertebrate kinetochore proteins. The following techniques have been used to characterize kinetochore assembly requirements. In addition, we describe assays that we utilized to identify factors that promote maintenance of preassembled kinetochores and those that induce kinetochore disassembly.
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序