Fluorescence Resonance Energy Transfer-Based Analysis of Lipoplexes
互联网
615
We describe three applications of the FRET technique to analysis of structural and thermodynamic properties of cationic lipids and their complexes with DNA. (1) Lipid mixing assay to determine the degree to which individual vesicles undergo fusion upon complex formation. (2) DNA binding assay to obtain cationic lipid-DNA binding curves for thermodynamic analysis or binding stoichiometry characterization. (3) DNA spacing assay to determine changes in DNA packing in the multilamellar lipoplex particle.
![四[N-邻苯二甲酰-(S)-叔亮氨酸基]二铑双(乙酸乙酯)加合物;154090-43-4;Elemental analysis(Nitrogen) 3.30 to 4.50 %;V66234-25mg](https://img1.dxycdn.com/p/s14/2025/1029/004/8672158669127143891.jpg!wh200)
